Lipid Extraction for Determination of trans-Fatty Acid in Food

Trans-fatty acids have been associated with coronary heart diseases. In this Short Note, the determination of trans-fatty acids using the SpeedExtractor E-916 for the extraction of the lipid of foodstuff is shown. The results obtained with SpeedExtractor E-916 were compared to the ones obtained with conventional methods (BUCHI Extraction Unit E-816 Hot Extration [1], Acid Hydrolysis [2], and Folch [3]). The results showed good correlation and it was demonstrated that neither oxidative degeneration nor new trans isomerization of unsaturated fatty acid occurred during the pressurized solvent extraction.