Separation of glycerides by Flash chromatography
Separation of glycerides by flash chromatography
Triglycerides are fatty acids present as esters in combination with glycerol. Tristearin, a glyceryl ester of stearic acid, is a simple triglyceride having three identical acyl chains. It is found in both plants and animals and may be used as a drug delivery vehicle for target drug compounds. This application demonstrates purification of a mixture of glycerides (mono-, di-, and tristearin) using C18 and amino phase chemistries. The C18 uses hydrophobic interaction to determine separation, wheras the amino phase uses lipophilic interaction between the stationary phase and the fatty acyl chain of the analyte to determine separation. The amino phase cartridge can be used in a normal phase or a reversed phase mode, depending on the solvents employed. When used in normal phase mode, the less polar compounds elute first, followed by the elution of the polar one’s selectivity.
