Nitrogen Determination in Pharmaceutical Active Peptides
Nitrogen and total protein determination in pharmaceutical products are described in the European Pharmacopoeia (Ph.Eur.) section2.5.9 and 2.5.33 method 7 [1, 2]. In the Pharmacopoeia of the United States (USP), nitrogen determination is described in section461 [3]. Therein, different titration techniques are specified.The Ph.Eur.describesback titration in the presence of the indicator methyl red mixed solution and the USP potentiometric boric acid titration.
TKN determination in water and wastewater
SpeedDigester K-439, Kjel Line and MultiDist: Steam distillation and Potentiometric determination of Total Kjeldahl Nitrogen (TKN) according to the kjeldahl method in water and wastewater along with determination of Limit of Detection (LOD) and Limit of Quantification (LOQ).
Sulfated Ash Determination for Pharmaceutical Quality Control
The wet digestion is used to remove all carbonous material from the sample. The remaining inorganic content can be weighed out, resulting in the sulfated ash, or then be used for further elemental analysis. The digestion is performed using concentrated sulfuric acid while heating the mixture. The heat is ramped automatically using the BUCHI Wet Digester B-440. Once the sulfuric acid has digested the sample and is evaporated, the sample is eventually ashed in a muffle oven to remove any remaining organic material.