Micro-Kjeldahl: Protein determination in Dairy Products
SpeedDigester K-439, Kjel Line and MultiDist: nitrogen and protein determination using micro-Kjeldahl method
An easy and reliable method for the determination of the Total Kjeldahl Nitrogen (TKN) and protein content in dairy products is introduced. The samples are digested using the SpeedDigester K-439 with 100 mL micro-Kjeldahl tubes. The steam distillation and titration in boric acid are performed with the MultiKjel in combination with a Metrohm Eco Titrator. This application note No. 872/2025 complies with official methods e.g. AOAC 991.20 and ISO 8968-2. Micro-Kjeldahl minimizes the amount of sulfuric acid and catalyst for the digestion, and the amount of sodium hydroxide for the distillation step as well. The savings in chemicals provides an economic and ecologic advantage to the classic Kjeldahl method while maintaining its high accuracy and preciseness.
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