Protein determination in animal feed using colorimetric titration
Protein determination in animal feed according to ISO 5983-2 and AOAC 2001.11 by digestion with Kjeldahl tablets and hydrogen peroxide followed by colorimetric titration using Optrode
A reliable and efficient method for the determination of total nitrogen and protein in animal feed according to ISO 5983-2 and AOAC 2001.11 is presented. The samples were digested using Kjeldahl tablets, hydrogen peroxide and the KjelDigester K-449. Distillation and boric acid titration were performed with the KjelMaster System K-375 / K-376 equipped with the colorimetric sensor Optrode. According to the norms, bromocresol green / methyl red mixed indicator was added to the boric acid for the automized colorimetric titration.